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Cell culture TC1 clone 6 (TC16) cells [American Type Tissue Culture Collection (ATCC), #CRL-2934, Manassas, VA] were cultured in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, 15 mM HEPES, 0.1 mM non-essential amino acids, 0.02% BSA, 15 mg/L sodium bicarbonate, 2 mg/L glucose

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